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Right after sequencing a brand new genome for The 1st time, purposeful protein-coding genes are initially determined by: BLAST queries that hunt for viral integration within the host genome Bioinformatic searches that reveal consensus sequences in expressed genes Aligning expressed exons sequenced from cDNA applying transcriptomics B and C A and B A lot of locations of non-coding eukaryotic DNA Beforehand thought to be "junk" at the moment are recognized to contain sequence things important to regulating gene expression.
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